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Efficient Optimization of CHO Cell Culture Medium and Feed for Increased Antibody Production
Sponsored by GE HealthCare Technologies
Traditional medium optimization strategies are labor intensive, costly, and time consuming. In this project, high-throughput screening technology was adopted along with statistical design to reduce costs and decrease time for optimizing culture conditions for maximized antibody production from a custom Chinese hamster ovary (CHO) cell clone.
The presented work includes two phases: phase 1 for basal medium optimization and phase 2 for feed optimization. Phase 1 and phase 2 each have two rounds. A DoE screening methodology was used to perform the round 1 experiments to identify which prototype medium and feed are having the greatest effect on cell growth and productivity. In round 2, a traditional optimization method in 125 mL shaker flask was used to further optimize the top conditions from the round 1 studies.
The use of a well-balanced variety of methods allowed optimization and formulation of chemically defined cell culture medium and feed for a recombinant CHO cell suspension culture with increased maximum cell density and productivity in a relatively short amount of time. In this case, a savings of 50% of the original scoped timeline was achieved. Productivity levels near 3.7 g/L were obtained with optimized conditions.
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