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Consistency in Peptone Manufacturing: Modern Methods Make a Difference

David Miller

August 10, 2017

1 Min Read

Solabia-Biotech-logo-300x181.gifWhat factors impact peptone variability? Variability can be linked to at least two critical elements: the biological raw materials and the production site. Raw materials can vary by species of animal or plant sourced, type of components (specific animal tissues as opposed to standardized materials), and geographical origins. Feeding practices also can cause variability. Within a single manufacturer, interbatch variability can be linked to the condition and age of a production site, its level of automation and sophistication, optimized production protocols, the types of products being processed, and the amount of human intervention.

Tangible Demonstrations of Consistency
Appropriate demonstrations of consistency can be found in batch reviews of actual productions. Microbial models can prove challenging in light of variables in media composition and the particularity of the microorganisms themselves. Total nitrogen is especially pertinent because it represents the main objective of the peptone: its contribution in nitrogen. An analysis of total nitrogen from 36 recent batches of Tryptone (a pancreatic digest of casein and the Solabia reference A1431) and 23 batches of Papaic Soy Peptone (Solabia reference A1607) using the Kjeldahl method are presented here, including calculations of the mean, standard deviation (SD), and coefficient of variation (CV). Additional data on the same sample pools using pH and sulfuric ash as measurable, recognizable points are included (Table 1).

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