A Transient Cell Line To Produce rAAV With Low-level hcDNA Encapsidation

The landscape of recombinant adeno-associated virus (rAAV) manufacturing is witnessing a transformative shift, where stable producer cell lines are emerging as the cell factories of the future. However, the move towards producer cell lines is a nuanced journey, requiring the use of transient transfection cell lines where flexibility and speed are of essence.

To address these needs, we developed a novel human embryonic kidney (HEK) 293 suspension cell line, the ELEVECTA™ transient cell line, designed to seamlessly integrate into various research workflows or large-scale GMP production. Here we demonstrate its capability for efficient and scalable production of rAAVs across multiple serotypes with low levels of encapsidated host cell DNA (hcDNA).