BPI Contributor

August 25, 2023

20 Min View
Scalable AAV Vector Purification Workflow Using AEX and Mixed-Mode Chromatography

Date: Aug 25, 2023

Duration: 20 Min

This webcast features: William H. Rushton, MS, Process Chromatography Scientist, Bio-Rad Laboratories.

Recombinant adeno‐associated viruses (rAAV) are among the most promising vectors for long‐term gene transduction. These viruses have a high degree of safety, making them ideal for gene therapy applications. Significant progress has been made in improving rAAV vector production and purification.

In this study, different chromatography workflow solutions were explored to purify rAAV8. Experimental conditions and data will be presented on using anion exchange (AEX) and mixed-mode chromatography to purify rAAV8.

Key Takeaways:

  • Increased efficiencies using AEX for capture.

    • Mixed-mode chromatography used to separate empty capsid from full capsid and remove DNA.

    • More flexible, economical purification workflow compared to traditional purification process.

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