Small Scale Escherichia Coli Cultivation Using BioBLU Single-Use and Reusable Vessels: A Comparative Study

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14-7-Eppendorf-logoIn recent years, single-use bioreactors have gained more importance in animal and human cell culture. With its new line of BioBLU frigid-wall, stirred-tank, single-use vessels, Eppendorf offers premium solutions for microbial applications. In the following case study, reproducible process control was achieved with parallel-operated BioBLU 0.3f single-use and reusable glass vessels, both used in an Eppendorf DASbox® mini bioreactor system. Fermentation of Escherichia coli K12 led to very comparable results, thus proving tested single-use vessels to be an appropriate tool to accelerate microbial process development and shorten time-to-market in all industries related to microbial production processes.

BioBLU of 0.3f single-use vessel (left) and DASbox mini bioreactor (right)

BioBLU of 0.3f single-use vessel (left) and DASbox mini bioreactor (right)

IntroductIon

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