Voices of Biotech
Podcast: MilliporeSigma says education vital to creating unbreakable chain for sustainability
MilliporeSigma discusses the importance of people, education, and the benefits of embracing discomfort to bolster sustainability efforts.
November 20, 2015
Date: Nov 19, 2015
Duration: 20 Min
Sponsored by Wacker
(c) Strandperle www.strandperle.biz
This webcast features: Dr. Andreas Anton, Director Bioprocess Development and Dr. Sebastian Schuck, Senior Manager Business Development, Wacker Biotech
While WACKER’s own technology ESETEC® has proven highly efficient in producing soluble proteins and antibody fragments via secretion, poorly soluble substances form aggregated inclusion bodies within the cell, which contain incorrectly and/or incompletely folded target proteins. Recently Wacker Biotech introduced FOLDTEC®, its novel refolding technology for bioengineered pharmaceutical proteins. With this new technology, biopharmaceuticals that tend to aggregate can be efficiently produced in their soluble-active form in high yields. The proprietary process utilizes specifically developed and optimized bacterial strains and a patented, antibiotic-free expression system. Furthermore, Wacker Biotech offers years of experience in the iterative screening of optimum refolding conditions in order to convert the insoluble target-protein aggregates into the biologically active form.
The potential of this new technology will be demonstrated in a recent case study on recombinant expression and in vitro refolding of thrombin, a key protease in the coagulation cascade. Thrombin is a difficult-to-manufacture enzyme used in surgery and other medical applications to control bleeding. Thanks to the FOLDTEC® toolbox, space/time yields have been increased successfully by 20-fold!
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