Voices of Biotech
Podcast: MilliporeSigma says education vital to creating unbreakable chain for sustainability
MilliporeSigma discusses the importance of people, education, and the benefits of embracing discomfort to bolster sustainability efforts.
Sponsored by GE HealthCare Technologies
For autologous T cell therapy to be effective, T cells sampled from a patient need to be grown in sufficient quantity before being injected back. The typical cell number required for an 80 kg patient is 8.109 cells. Achieving such a dose with T flasks, while maintaining cell health, poses logistical challenges that can become intractable when the number of patients rises. This is why the Xuri™ Cell expansion system W5, a rocking platform which enables automated high cell density culture in a close environment, is used instead of conventional bioreactors. After a 14 day culture with the Xuri W5, cell concentrations of 1.107cells/mL in a 1 L volume have been consistently reached and cells exhibited a healthy phenotype. These experiments have been performed with rocking speed and angle set at 10 rpm, and 6° respectively. In the study presented here, we investigate the influence of these parameters on the cell growth kinetics and health in order to select the settings that optimize T cell growth, thereby allowing for faster expansion.
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