Characterization and Engineering Performance of the Allegro™ STR 2000 Single-Use Stirred Tank Bioreactor

View PDF

Pall_rsz_1289934Process performance in bioreactors is strongly influenced by the efficiency of bulk fluid mixing and the oxygen mass transfer coefficient (kLa). The success of traditional stainless steel STR systems lies in their direct impeller driven agitation that can deliver a wide range of specific power inputs to the fluid. The Allegro™ range of single-use stirred tank bioreactors has adopted this direct driven impeller technology, which allows a wide range of specific power inputs to be achieved. In addition, modifications to current design features utilized with other single-use bioreactor technologies in the market place today, have been implemented for increased ease of use and process assurance.

This white paper discusses how the Allegro STR range has been designed to provide a predictable scale-up performance modeling, therefore, the comparison of the mixing and mass transfer capabilities across the bioreactor range is presented with consistency from reactor to reactor at specified volume sizes. This information can help significantly in the selection of operating parameters during process transfer, including scale-up and scaledown, from bench-scale and stainless steel reactors. This leads to a reduction in the time required for additional development that may be needed for internal engineering performance modeling, and aids with conceptual understanding of how the cell line may perform during the process. This information can help significantly in the selection of operating parameters during process transfer, including scale-up and scale-down, from bench-scale and stainless steel reactors.

Leave a Reply