Downstream

Purification of Antibody Fragments with Amsphere™ A3 Protein A Resin

This webcast features: Gerald Platteau, Application Development Engineer, JSR Life sciences Purification of full-length antibodies containing an Fc region relies on Protein A affinity chromatography as the golden standard capture step. The well-known robustness, high selectivity and scalability make it an ideal platform technique, minimizing downstream processing development time to bring new antibodies to market. After monoclonal antibodies (mAbs), various forms of antibody fragments are now becoming the next most important class of protein based therapeutics. These antibody fragments offer…

CHT Column Packing is Easier
Than You Think

This webcast features: Dr. Mark Snyder, Manager, R&D Applications, Process Chromatography, Bio-Rad Laboratories This webcast presents best practices and guidelines for packing CHT™ Ceramic Hydroxyapatite Media at process scale. CHT, is an easy-to-use mixed-mode chromatography media. It offers cation exchange and affinity interactions. Unlike other chromatography media, CHT is both the ligand and the support matrix. It is used for the purification of numerous types of biomolecules and is considered the gold standard for aggregate removal. This webcast highlight CHT…

Exchange Your Buffer Exchange

This webcast features: Quincy Mehta, Product Manager, Unchained Labs Buffer preparation and buffer exchange are tedious, manual processes that take a lot of time, particularly, hands-on time. This ultimately slows down your development timelines. In this webinar, you will learn how Unchained Labs breaks the manual buffer exchange bottleneck with Grunt, including: Formulation screening and development to scale-up Buffer preparation, exchange and analysis in a single bench-top instrument Impeccable mass recovery and time savings Analytical data supports protein handling is…

Impact of Single-Use Tangential Flow Filtration in Downstream Bioprocessing

This webcast features: Michael LaBreck, Sales Director, Repligen TagenX Products Single-use solutions are fast becoming platform in bioprocessing as a response to pressure to optimize process economics and process efficiencies. Implementing single-use alternatives in tangential flow filtration (TFF), a critical step in downstream bioprocessing for concentration and diafiltration (buffer exchange), is a major part of this transformation. Repligen TangenX™ SIUS™ Single-use Cassettes are the first purpose-built cassettes designed for tangential flow filtration. This webinar will demonstrate how deploying these innovative…

Effective Deployment of OPUS Pre-Packed Chromatography Columns for Bench-Scale Process Development and Process Validation

This webcast features: Tim Schroeder, Director, Product Management at Repligen GmbH Formerly Atoll pre-packed columns, OPUS® pre-packed chromatography columns for process development are used in all steps of downstream development, including screening, process validation and viral clearance, scale up and sample preparation. This webcast will demonstrate how OPUS® ValiChrom Columns, glass pre-packed columns that are exact scale-down models of corresponding full-scale chromatography columns, are ideal for process validation including viral clearance. It will also demonstrate how OPUS® RoboColumns®, miniaturized columns…

Ultimate Flexibility from Pre-Packed to Unpacked: OPUS 45R and OPUS 60R Columns

This webcast features: Fletcher Malcom, Director of Product Management, Repligen With an innovative side port that allows for easy resin unpacking, OPUS® 45R and OPUS® 60R Columns provide the ultimate flexibility in pre-packed column technology. Without compromising chromatographic performance or cleanability, the new feature helps mitigate the risk of implementing pre-packed columns in CGMP settings, and allows for re-use of the unpacked resin in other columns. This webinar covers test results on chromatographic performance, bioburden testing, shipping tests and simple…

Enabling Custom Solutions for Downstream Processing for Future Therapies: AAV Case Study

This webcast features: Orjana Terova, Purification Product Manager, Thermo Fisher Scientific There is a need in the industry for a standard purification strategy for the manufacture of novel molecules including viral vectors and oligomeric therapies. Working with customers, Thermo Fisher Scientific has developed innovative purification resins through the POROS™ custom resin program and CaptureSelect™ ligand technology. This combination of ligand and resin development has allowed for development of platform purification processes that increase productivity and improve product yield for gene therapies.…

Continuous Chromatography of MAbs Using BioSC: Process Design and Regulatory Considerations

This webcast features: Vincent Monchois, Strategic Project Director, Biopharma, Novasep Challenges in bio-manufacturing up to commercial stage include improving lead times, securing the process, improving productivity, and reducing COGs. The implementation of a continuous manufacturing process is a viable solution to address these challenges. The chromatography step represents one of the key stages of a manufacturing process for (bio)pharmaceutical compounds. Continuous chromatography was originally developed by the Petroleum industry in the 1960s and has been successfully implemented in the Food-processing…

Principles and Troubleshooting of Large Scale Column Packing

This webcast features: J. Kevin O’Donnell, PhD, Process Chromatography Support Manager, Tosoh Bioscience This presentation focuses on the packing of process scale chromatography columns. Including general principles of column packing, common packing techniques used in packing process scale columns, qualification and evaluation of the packed column, and the troubleshooting of issues common to large scale column packing. Watch this webcast now.

Simulation and Optimization Software for a Chromatographic Step

This webcast features: Thomas Flouquet, Area Sales Manager/Application Specialist for low pressure chromatography BioSC® Predict is the first simulation and optimization software for the development of chromatography processes dedicated to the purification of biopharmaceuticals. Its innovation was recognized at the Achema 2015 Awards in the Pharmaceutical Engineering category. With a few clicks, the user can easily generate batch, parallel batch or continuous chromatography recipes and directly run them on BioSC® technology, with any adsorption media. BioSC® Predict is driven by…