Author Archives: Renaud Jacquemart

Membrane Adsorbers, Columns: Single-Use Alternatives to Resin Chromatography

Filtration membranes are used extensively throughout the biopharmaceutical industry for a range of applications, from coarse filtration to nanofiltration. Advantages of filter technologies include easy scaling, disposability, and (for many membrane filters) rapid and robust performance in a single-pass. The same advantages have been realized with membrane adsorbers. Chromatography resins are inherently disadvantaged by diffusion limits of the pores in chromatography media. Therefore, resin columns must be significantly oversized to match the performance of high productivity bioreactors. By comparison, membrane…

NatriFlo® HD-Q Membrane Adsorber: A New Single-Use High-Capacity Polishing Tool with Excellent Salt Tolerance and Process Robustness for MAb Purification

Strong anion exchange (Q) chromatography has become an industry standard in MAb production. It is a proven technology to remove DNA, viruses, endotoxins and acidic host cell proteins from process feed streams in flowthrough mode. Recent trends show an increasing interest in downstream single-use technologies and flexible biomanufacturing due to advancement in cell culture technology and emergence of biosimilars. Traditional chromatography columns are slow, often oversized and not suitable for flexible biomanufacturing. Conventional membrane adsorbers cannot provide sufficient process robustness…

NatriFlo™ HD-Q: A High Performance Disposable Polishing Tool For MAb Purification

As biopharmaceutical developers embrace single-use technologies to reduce risk, improve process speed, efficiency and flexibility, they have found few options in downstream unit operations.  With the introduction of Natrix hydrogel technology, developers now find a chromatographic tool that provides the binding capacity of the best modern resins, but with ten times higher flow rates. This study illustrates the NatriFlo HD-Q anion exchange adsorber which provides high productivity polishing for monoclonal antibody for GMP manufacturing, including under high conductivity conditions. This…