Author Archives: BPI Contributor

Insulin Improves Cell Growth and Influenza Yield in a HEK293 Suspension Cell Line

This webcast features: Aziza Manceur, Research Officer, National Research Council Canada To produce large quantities of vaccines in a rapid and cost-effective way is the main challenge that the vaccine industry is facing. Major changes to current bioprocesses are difficult to implement due to costs associated with the regulatory approval process. Therefore, we propose to use insulin, a protein currently used and approved in several processes and production platforms, as an additive to boost cell growth and viral production. The HEK293SF-3F6 cell…

Continuous Capture Chromatography, A simple, Robust Approach to Move From Batch to Continuous Capture

Increasing demands for the production of biopharmaceuticals, such as monoclonal antibodies (mAbs), vaccines and recombinant proteins, require new, innovative purification approaches with higher efficiency and productivity while maintaining or reducing costs. One of these approaches is continuous capture chromatography. Continuous capture chromatography, also known as CaptureSMB was developed by ChromaCon AG. CaptureSMB is a 2-column periodic counter current chromatography (2-PCC) process where the first column is fully loaded and breaks through to the second column. The first column is eluted…

Exchange Your Buffer Exchange

This webcast features: Quincy Mehta, Product Manager, Unchained Labs Buffer preparation and buffer exchange are tedious, manual processes that take a lot of time, particularly, hands-on time. This ultimately slows down your development timelines. In this webinar, you will learn how Unchained Labs breaks the manual buffer exchange bottleneck with Grunt, including: Formulation screening and development to scale-up Buffer preparation, exchange and analysis in a single bench-top instrument Impeccable mass recovery and time savings Analytical data supports protein handling is…

Gram Scale Transient Antibody Production and Stable Cell Line Generation Using Flow Electroporation™ Technology

In recent years researchers have turned to transient gene expression (TGE) as an alternative to CHO stable cell line production for early stage antibody development. Despite advances in transfection methods and culture optimization, the majority of CHO-based TGE systems produce insufficient antibody titers (low mg/L level) for full use within the biotherapeutic development pipelines. MaxCyte’s delivery platform for cell engineering is based on Flow Electroporation™ technology and provides a universal means of fully scalable, highly efficient CHO-based TGE for the…

Single-Use Technology: The Next 5 Challenges to Conquer

Now that single-use technology plays a part in nearly all bio-production processes, what is next? Several challenges remain that are still serious enough to delay or even stop the use of single-use technology, which in turn can extend the time to market and increase the cost of lifesaving biopharmaceutical products. Of the existing difficulties, which five are the most critical? This paper considers five existing challenges in the implementation of single-use technology including scale, automation, product compatibility and compliance, speed…

KBI Biopharma Acquires Alliance Protein Laboratories, Expands Biophysical Characterization Capabilities

KBI Biopharma, Inc. (KBI) has agreed to acquire the assets of San Diego-based Alliance Protein Laboratories (Alliance), a leading analytical services company specializing in biophysical characterization of biopharmaceuticals. Founded in 1998, Alliance is well known and highly regarded around the world for its expertise in characterization of therapeutic proteins, peptides, nucleic acids and vaccines. Recognized as the foremost expert in analytical ultracentrifugation (AUC), an essential technology for determining physical stability, association state, molecular weight and quantitative characterization of protein aggregates,…

JSR Life Sciences Acquires Cell Line Developer Selexis SA, Integrates With KBI Biopharma, Inc.

JSR Life Sciences (JLS) announced today that JSR Corporation has agreed to acquire Selexis SA, a pioneering life sciences company and global leader in mammalian (suspension-adapted CHO-K1) cell-line generation technologies. Once completed, this transaction will mark the second major biotechnology addition to the JSR group of companies following JSR Corporation’s acquisition of KBI Biopharma in 2015. Selexis will be integrated within KBI Biopharma’s operations to create the most robust and fastest “Gene to GMP” service offering in the biopharmaceutical industry.…

Rentschler Biotechnologie and Rentschler Fill Solutions Announce Strategic Partnership for One-Stop Solutions with New State-of-the-Art Fill and Finish Facilities

Rentschler Biotechnologie GmbH, a leading contract development and manufacturing organization (CDMO) for biopharmaceuticals, and Rentschler Fill Solutions GmbH, an independent specialist for aseptic fill and finish services, today announced a strategic partnership to provide new state-of-the-art fill and finish facilities and one-stop solutions for biopharmaceutical products to meet the needs of Rentschler Biotechnologie’s clients. Under the terms of the agreement, Rentschler Fill Solutions will prospectively serve as the exclusive partner for the fill and finish services of Rentschler Biotechnologie’s manufacturing…

Eppendorf Introduces the New BioFlo® 120 Bioprocess Control Station

Leading life science company Eppendorf introduces the BioFlo 120, their latest bench scale fermentor/bioreactor system for research and development. This flexible instrument is capable of microbial fermentation as well as mammalian cell culture applications with a single platform. It features an extensive range of glass and BioBLU® Single-Use Vessel options (250 mL – 40 L). Universal connections for digital Mettler Toledo® ISM and analog sensors make it easy to monitor a variety of critical process parameters. “We know that in…

Sartorius Stedim Biotech Launches ambr® 15 Bioreactor System with Nova BioProfile® FLEX2 Integration

Sartorius Stedim Biotech (SSB), a leading international supplier for the biopharmaceutical industry, announced that the ambr® 15 automated micro bioreactor system combined with a Nova Biomedical (Nova) BioProfile® FLEX2 automated cell culture analyzer is on show for the first time at the Cell Line Development & Engineering Conference in San Diego, California. This newly integrated system, which is a first from SSB and Nova’s successful collaboration, enables rapid at-line sample collection and analysis of massive quantities of key cell culture…