Author Archives: Kristina Nilsson-Valimaa

Polishing a Monoclonal Antibody (MAb) Using Capto™ adhere ImpRes in Bind and Elute Mode

A platform approach for MAb purification is desirable as it saves both time and money in process development. GE Healthcare Life Sciences’ MAb toolbox employs protein A chromatography media (resins) as well as a wide range of options for polishing purification steps. One of these options is Capto adhere ImpRes, a cost-effective and flexible multimodal anion exchange chromatography medium designed for high-resolution polishing of MAbs. The medium allows operation in either bind-elute (B/E) or flow-through (FT) modes. Here we describe…

Rapid and Scalable Microplate Development of a Two-Step Purification Process

    High-throughput screening and process development methods are becoming more widely used in the biopharmaceutical industry. Recent development of high-expression (high–target-titer) recombinant culture methods has enhanced the need to also develop more effective separation products, methods, and processes (1). Part of the solution would be chromatographic resins offering higher capacities and flow rates.       However, developing an optimized purification process that involves several chromatographic steps can consume significant time and samples. In addition, a purification process developed…