BPI Contributor

October 20, 2017

20 Min View
Targeted Locus Amplification for Transgene Sequencing in rCHO Clones Expressing Therapeutic Lysosomal Enzyme

Date: Oct 20, 2017

Duration: 20 Min

This webcast features: Vishal Agrawal, PhD, Scientist II, Cell and Molecular Biology, Biomarin Pharmaceutical Inc. and Joseph Abad, Field Applications Scientist, MaxCyte.

Transgene site of integration dictates clonal stability and the long term transcriptional activity of gene of interest in recombinant CHO cells. In this presentation, we will discuss the application of targeted locus amplification (TLA) technology to identify genomic sites of transgene integration and integrity of transgene sequence itself to assess the suitability of recombinant CHO clones as a candidate for generating candidate therapeutic lysosomal enzyme for pre-clinical studies.

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