This webcast features: Dr. Mark Snyder, Manager, R&D Applications,
Process Chromatography, Bio-Rad Laboratories
This webcast presents best practices and guidelines for packing CHT™ Ceramic Hydroxyapatite Media at process scale. CHT, is an easy-to-use mixed-mode chromatography media. It offers cation exchange and affinity interactions. Unlike other chromatography media, CHT is both the ligand and the support matrix. It is used for the purification of numerous types of biomolecules and is considered the gold standard for aggregate removal.
This webcast highlight CHT Media transfer methodologies and column packing guidelines. CHT is incompressible and has high specific gravity, a rapid settling rate, and sensitivity to mechanical shear. These variations from traditional compressible resins have to be considered when designing CHT column-packing protocols. In this webcast, expert Dr. Mark Snyder provides recommendations and guidelines for successful CHT column packing.
You will learn:
- Slurry preparation techniques
- Slurry transfer methodologies
- Column packing guidelines
- Packed column evaluation and qualification testing
- Post-packing considerations